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Figure 2. Differential TNF production due to stimulation thresholds and TNF trafficking (A) CD3 expression on Pan T cells and NCR1 or CD16 expression on NK cells from 6 donors were measured by flow staining to determine ABC. (B) Immunoblot of lysates of Pan T cells or NK cells from 6 donors stimulated with immobilized anti-CD3 or anti-NCR1 and anti-CD16, respectively, for 16 h in the presence of GolgiPlug. The ratio of TNF/actin from each donor was calculated. (C) Immunoblot of lysates of Pan T cells or NK cells from 6 donors stimulated with PMA/ionomycin in the presence of transporter inhibitors (Ti). The ratio of TNF/ actin from each donor was calculated. (D) TNF in the cell culture supernatants of Pan T cells or NK cells from 8 different donors at 4 h post-stimulation. (E–G) Surface staining of TNF on Pan T or NK cells from 6 donors in the presence of DMSO or GW280264X post-4 h PMA/ionomycin stimulation. (E) Repre- sentative staining, (F) frequency of TNF+ among Pan T or NK cells, and (G) mean fluorescence intensity (MFI) of TNF+ among Pan T or NK cells. (H) Immunoblot of lysates of Pan T cells or NK cells from 7 donors analyzed with <t>anti-ADAM17</t> and anti-actin. The ratio of ADAM17/actin from each donor was calculated. Data are presented as means ± SEM (A). p values were determined by one-way ANOVA followed by Tukey’s multiple comparisons test (A and B) or a paired two- tailed t test for two-group comparisons (C, D, F, G, and H); *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.
Adam17, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 2. Differential TNF production due to stimulation thresholds and TNF trafficking (A) CD3 expression on Pan T cells and NCR1 or CD16 expression on NK cells from 6 donors were measured by flow staining to determine ABC. (B) Immunoblot of lysates of Pan T cells or NK cells from 6 donors stimulated with immobilized anti-CD3 or anti-NCR1 and anti-CD16, respectively, for 16 h in the presence of GolgiPlug. The ratio of TNF/actin from each donor was calculated. (C) Immunoblot of lysates of Pan T cells or NK cells from 6 donors stimulated with PMA/ionomycin in the presence of transporter inhibitors (Ti). The ratio of TNF/ actin from each donor was calculated. (D) TNF in the cell culture supernatants of Pan T cells or NK cells from 8 different donors at 4 h post-stimulation. (E–G) Surface staining of TNF on Pan T or NK cells from 6 donors in the presence of DMSO or GW280264X post-4 h PMA/ionomycin stimulation. (E) Repre- sentative staining, (F) frequency of TNF+ among Pan T or NK cells, and (G) mean fluorescence intensity (MFI) of TNF+ among Pan T or NK cells. (H) Immunoblot of lysates of Pan T cells or NK cells from 7 donors analyzed with <t>anti-ADAM17</t> and anti-actin. The ratio of ADAM17/actin from each donor was calculated. Data are presented as means ± SEM (A). p values were determined by one-way ANOVA followed by Tukey’s multiple comparisons test (A and B) or a paired two- tailed t test for two-group comparisons (C, D, F, G, and H); *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.
Crystal Structure Of The Tace Lisinopril Complex, supplied by Accelrys, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 2. Differential TNF production due to stimulation thresholds and TNF trafficking (A) CD3 expression on Pan T cells and NCR1 or CD16 expression on NK cells from 6 donors were measured by flow staining to determine ABC. (B) Immunoblot of lysates of Pan T cells or NK cells from 6 donors stimulated with immobilized anti-CD3 or anti-NCR1 and anti-CD16, respectively, for 16 h in the presence of GolgiPlug. The ratio of TNF/actin from each donor was calculated. (C) Immunoblot of lysates of Pan T cells or NK cells from 6 donors stimulated with PMA/ionomycin in the presence of transporter inhibitors (Ti). The ratio of TNF/ actin from each donor was calculated. (D) TNF in the cell culture supernatants of Pan T cells or NK cells from 8 different donors at 4 h post-stimulation. (E–G) Surface staining of TNF on Pan T or NK cells from 6 donors in the presence of DMSO or GW280264X post-4 h PMA/ionomycin stimulation. (E) Repre- sentative staining, (F) frequency of TNF+ among Pan T or NK cells, and (G) mean fluorescence intensity (MFI) of TNF+ among Pan T or NK cells. (H) Immunoblot of lysates of Pan T cells or NK cells from 7 donors analyzed with <t>anti-ADAM17</t> and anti-actin. The ratio of ADAM17/actin from each donor was calculated. Data are presented as means ± SEM (A). p values were determined by one-way ANOVA followed by Tukey’s multiple comparisons test (A and B) or a paired two- tailed t test for two-group comparisons (C, D, F, G, and H); *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.
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Network graphs of different outcomes: (A) CD3 + ; (B) CD4 + ; (C) CD8 + ; (D) CD4 + /CD8 + ; (E) NK; (F) clinical response rate; (G) 6-month survival; (H) 1-year survival; (I) 2-year survival. The width of the lines in the network graph is proportional to the number of RCTs used for the comparisons, and the node sizes correspond to the total sample sizes for the treatments. The clinical effective rate graph F was made with R3.6.3. TACEADI, <t>TACE+Aidi</t> injection; TACECKSI, TACE+Compound Kushen injection; TACEBOEI, TACE+ Bruisea Oil Emulsion injection; TACEKAI, TACE+Kangai injection; TACEHCSI, TACE+Huachansu injection; TACEKLTI, TACE+Kanglaite injection; TACESQFZI, TACE+Shenqi Fuzheng injection; <t>TACEXAPI,</t> <t>TACE+Xiaoaiping</t> injection.
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Network graphs of different outcomes: (A) CD3 + ; (B) CD4 + ; (C) CD8 + ; (D) CD4 + /CD8 + ; (E) NK; (F) clinical response rate; (G) 6-month survival; (H) 1-year survival; (I) 2-year survival. The width of the lines in the network graph is proportional to the number of RCTs used for the comparisons, and the node sizes correspond to the total sample sizes for the treatments. The clinical effective rate graph F was made with R3.6.3. TACEADI, <t>TACE+Aidi</t> injection; TACECKSI, TACE+Compound Kushen injection; TACEBOEI, TACE+ Bruisea Oil Emulsion injection; TACEKAI, TACE+Kangai injection; TACEHCSI, TACE+Huachansu injection; TACEKLTI, TACE+Kanglaite injection; TACESQFZI, TACE+Shenqi Fuzheng injection; <t>TACEXAPI,</t> <t>TACE+Xiaoaiping</t> injection.
Drug Eluting Bead Transarterial Chemoembolization (Deb Tace), supplied by Weinmann GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 2. Differential TNF production due to stimulation thresholds and TNF trafficking (A) CD3 expression on Pan T cells and NCR1 or CD16 expression on NK cells from 6 donors were measured by flow staining to determine ABC. (B) Immunoblot of lysates of Pan T cells or NK cells from 6 donors stimulated with immobilized anti-CD3 or anti-NCR1 and anti-CD16, respectively, for 16 h in the presence of GolgiPlug. The ratio of TNF/actin from each donor was calculated. (C) Immunoblot of lysates of Pan T cells or NK cells from 6 donors stimulated with PMA/ionomycin in the presence of transporter inhibitors (Ti). The ratio of TNF/ actin from each donor was calculated. (D) TNF in the cell culture supernatants of Pan T cells or NK cells from 8 different donors at 4 h post-stimulation. (E–G) Surface staining of TNF on Pan T or NK cells from 6 donors in the presence of DMSO or GW280264X post-4 h PMA/ionomycin stimulation. (E) Repre- sentative staining, (F) frequency of TNF+ among Pan T or NK cells, and (G) mean fluorescence intensity (MFI) of TNF+ among Pan T or NK cells. (H) Immunoblot of lysates of Pan T cells or NK cells from 7 donors analyzed with anti-ADAM17 and anti-actin. The ratio of ADAM17/actin from each donor was calculated. Data are presented as means ± SEM (A). p values were determined by one-way ANOVA followed by Tukey’s multiple comparisons test (A and B) or a paired two- tailed t test for two-group comparisons (C, D, F, G, and H); *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.

Journal: Cell reports. Medicine

Article Title: Characterization and comparative analysis of multifunctional natural killer cell engagers during antitumor responses.

doi: 10.1016/j.xcrm.2025.102117

Figure Lengend Snippet: Figure 2. Differential TNF production due to stimulation thresholds and TNF trafficking (A) CD3 expression on Pan T cells and NCR1 or CD16 expression on NK cells from 6 donors were measured by flow staining to determine ABC. (B) Immunoblot of lysates of Pan T cells or NK cells from 6 donors stimulated with immobilized anti-CD3 or anti-NCR1 and anti-CD16, respectively, for 16 h in the presence of GolgiPlug. The ratio of TNF/actin from each donor was calculated. (C) Immunoblot of lysates of Pan T cells or NK cells from 6 donors stimulated with PMA/ionomycin in the presence of transporter inhibitors (Ti). The ratio of TNF/ actin from each donor was calculated. (D) TNF in the cell culture supernatants of Pan T cells or NK cells from 8 different donors at 4 h post-stimulation. (E–G) Surface staining of TNF on Pan T or NK cells from 6 donors in the presence of DMSO or GW280264X post-4 h PMA/ionomycin stimulation. (E) Repre- sentative staining, (F) frequency of TNF+ among Pan T or NK cells, and (G) mean fluorescence intensity (MFI) of TNF+ among Pan T or NK cells. (H) Immunoblot of lysates of Pan T cells or NK cells from 7 donors analyzed with anti-ADAM17 and anti-actin. The ratio of ADAM17/actin from each donor was calculated. Data are presented as means ± SEM (A). p values were determined by one-way ANOVA followed by Tukey’s multiple comparisons test (A and B) or a paired two- tailed t test for two-group comparisons (C, D, F, G, and H); *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.

Article Snippet: Membranes were blocked in 5% milk and incubated with primary antibodies: TNF (Cell signaling, Cat# 6945), ADAM17 (Cell Signaling, Cat# 3976) and β-actin (Cell Signaling, Cat# 3700), followed by HRP-coupled secondary antibodies.

Techniques: Expressing, Staining, Western Blot, Cell Culture, Fluorescence, Two Tailed Test

Network graphs of different outcomes: (A) CD3 + ; (B) CD4 + ; (C) CD8 + ; (D) CD4 + /CD8 + ; (E) NK; (F) clinical response rate; (G) 6-month survival; (H) 1-year survival; (I) 2-year survival. The width of the lines in the network graph is proportional to the number of RCTs used for the comparisons, and the node sizes correspond to the total sample sizes for the treatments. The clinical effective rate graph F was made with R3.6.3. TACEADI, TACE+Aidi injection; TACECKSI, TACE+Compound Kushen injection; TACEBOEI, TACE+ Bruisea Oil Emulsion injection; TACEKAI, TACE+Kangai injection; TACEHCSI, TACE+Huachansu injection; TACEKLTI, TACE+Kanglaite injection; TACESQFZI, TACE+Shenqi Fuzheng injection; TACEXAPI, TACE+Xiaoaiping injection.

Journal: Frontiers in Medicine

Article Title: Immune regulation and clinical response of Chinese herbal injections combined with TACE in hepatocellular carcinoma: a cumulative logit regression and Bayesian network meta-analysis

doi: 10.3389/fmed.2025.1567137

Figure Lengend Snippet: Network graphs of different outcomes: (A) CD3 + ; (B) CD4 + ; (C) CD8 + ; (D) CD4 + /CD8 + ; (E) NK; (F) clinical response rate; (G) 6-month survival; (H) 1-year survival; (I) 2-year survival. The width of the lines in the network graph is proportional to the number of RCTs used for the comparisons, and the node sizes correspond to the total sample sizes for the treatments. The clinical effective rate graph F was made with R3.6.3. TACEADI, TACE+Aidi injection; TACECKSI, TACE+Compound Kushen injection; TACEBOEI, TACE+ Bruisea Oil Emulsion injection; TACEKAI, TACE+Kangai injection; TACEHCSI, TACE+Huachansu injection; TACEKLTI, TACE+Kanglaite injection; TACESQFZI, TACE+Shenqi Fuzheng injection; TACEXAPI, TACE+Xiaoaiping injection.

Article Snippet: The network funnel plots suggest the presence of small sample effects between TACE and TACE+Xiaoaiping, TACE and TACE+Kanglaite, and TACE and TACE+Shenqi Fuzheng in peripheral blood T lymphocyte subsets and overall survival outcomes ( ).

Techniques: Injection, Emulsion

Funnel plot of pairwise comparison among each treatment on (A) CD3 + ; (B) CD4 + ; (C) CD8 + ; (D) CD4 + /CD8 + ; (E) NK; (F) 6-month survival; (G) 1-year survival; (H) 2-year survival. Note: TACEADI, TACE+Aidi injection; TACECKSI, TACE+ Compound Kushen injection; TACEBOEI, TACE+ Bruisea Oil Emulsion injection; TACEKAI, TACE+Kangai injection; TACEHCSI, TACE+Huachansu injection; TACEKLTI, TACE+Kanglaite injection; TACESQFZI, TACE+Shenqi Fuzheng injection; TACEXAPI, TACE+Xiaoaiping injection.

Journal: Frontiers in Medicine

Article Title: Immune regulation and clinical response of Chinese herbal injections combined with TACE in hepatocellular carcinoma: a cumulative logit regression and Bayesian network meta-analysis

doi: 10.3389/fmed.2025.1567137

Figure Lengend Snippet: Funnel plot of pairwise comparison among each treatment on (A) CD3 + ; (B) CD4 + ; (C) CD8 + ; (D) CD4 + /CD8 + ; (E) NK; (F) 6-month survival; (G) 1-year survival; (H) 2-year survival. Note: TACEADI, TACE+Aidi injection; TACECKSI, TACE+ Compound Kushen injection; TACEBOEI, TACE+ Bruisea Oil Emulsion injection; TACEKAI, TACE+Kangai injection; TACEHCSI, TACE+Huachansu injection; TACEKLTI, TACE+Kanglaite injection; TACESQFZI, TACE+Shenqi Fuzheng injection; TACEXAPI, TACE+Xiaoaiping injection.

Article Snippet: The network funnel plots suggest the presence of small sample effects between TACE and TACE+Xiaoaiping, TACE and TACE+Kanglaite, and TACE and TACE+Shenqi Fuzheng in peripheral blood T lymphocyte subsets and overall survival outcomes ( ).

Techniques: Comparison, Injection, Emulsion